Comprehensive Assessment of Transcorneal Permeation, Antimicrobial, and Antifungal Activities of Andrographolide-Loaded Nanosuspension: In vitro and In vivo Studies

This study aimed to comprehensively assess the transcorneal permeation, antimicrobial, and antifungal activities of a nanosuspension loaded with Andrographolide, promising herbal compound. Through combination in vitro vivo studies, efficacy, potential applications ocular drug delivery were investigated. In phase, permeation studies conducted using Franz diffusion cells excised rabbit corneas. The demonstrated significantly enhanced compared control formulation, indicating its ability effectively deliver Andrographolide through cornea into tissues. Additionally, exhibited potent antimicrobial against various pathogens, as determined by agar broth microdilution assays. Building upon results, performed model. Ocular tolerability was assessed eye observations, no observed signs irritation or adverse effects. Furthermore, nanosuspension's evaluated instilling formulation rabbits' eyes monitoring conjunctival congestion inflammation. results significant reduction congestion, highlighting for combating infections comprehensive assessment presented this establishes capability Andrographolide-loaded remarkable activities. These findings underscore an effective system inflammatory conditions. contributes development novel therapeutic approaches ophthalmology, aiming improve patient outcomes provide alternative treatment options diseases.
 Keywords: Transcorneal permeation; Nanosuspension; Andrographolide; Antimicrobial activity; Antifungal